Cloning, characterization, and transcriptional analysis of a gene encoding an alpha-crystallin-related, small heat shock protein from the thermophilic cyanobacterium Synechococcus vulcanus.

Cloning, Characterization, and Transcriptional Analysis of a Gene Encoding an a-Crystallin-Related, Small Heat Shock Protein from the Thermophilic Cyanobacterium Synechococcus vulcanus

Cloning and characterization of a small heat shock protein cDNA clone of Wuchereria bancrofti

A 426 bp cDNA encoding a predicted small heat shock protein (smHSP) was isolated from Wuchereria bancrofti lambda zap L3 cDNA expression library by immunoscreening with microfilaremic sera. The open reading frame of the cDNA clone encodes a predicted protein of 142 amino acids (aa), which had high sequence identity with other nematode smHSPs. The homologous regions conserved in several differen...

Nucleotide sequence of the psaC gene of the cyanobacterium Synechococcus vulcanus.

The psaC is found in chloroplast genomes of higher plants, tobacco (1), liverwort (2), barley (3), maize (4), pea (5) and wheat (5). It encodes a photosystem-I associated polypeptide, designated as Subunit VII, 9 kDa subunit, PsaC, etc., presumably the apoprotein for iron-sulfur clusters, Centers A and B. We previously reported a partial N-terminal amino acid sequence of Subunit VII of a thermo...

Cloning and molecular characterization of TaERF6, a gene encoding a bread wheat ethylene response factor

Ethylene response factor proteins are important for regulating gene expression under different stresses. Different isoforms for ERF have previously isolated from bread wheat (Triticum aestivum L.) and related genera and called from TaERF1 to TaERF5. We isolated, cloned and molecular characterized a novel one based on TdERF1, an isoform in durum wheat (Tri...

Cloning and Expression of Heat Shock Protein 60kDa Gene from Brucella melitensis as Subunit Vaccine

Brucellosis is caused by the bacterium Brucella and affects various domestic and wild species. GroEL (Heat Shock Protein 60kDa) as one of the major antigens that stimulate the immune system, increases Brucella survival. The aim of the current study was to clone and express GroEL in Escherichia coli in order to design subunit vaccine. Amplifying was performed using specific primers. The full-len...